Abstract：Objective: To explore the effect of Nox2 in human dermal fibroblasts damage induced by adriamycin. Methods: A human dermal fibroblasts injury model was established by exposure to adriamycin. Western blotting was used to detect Nox2 protein expression in the injury of human dermal fibroblasts induced by adriamycin. The cell survival rate was determined by MTT method. Superoxide dismutase (SOD) activity, malondialdehyde (MDA) and reactive oxygen species (ROS) levels were detected by biochemical kit. Moreover, the level of apoptosis was detected by Western blotting.Results: The relative expression of Nox2 in adriamycin group was higher than that in the control group, and the difference was statistically significant (P<0.05). Compared with adriamycin group, inhibition of Nox2 expression by siRNA decreased the level of MDA and ROS, as well as enhanced the activity of SOD (P<0.05). In addition, inhibition of Nox2 expression by siRNA decreased the apoptosis of human dermal fibroblasts induced by adriamycin (P<0.05). Conclusion: Targeting inhibition of Nox2 expression by siRNA can protect against the injury of human dermal fibroblasts induced by adriamycin, the mechanism is the reducing of oxidative stress and ROS level.
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2019, Vol. 25 
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