Abstract：Objective: To investigate the expressions of miR-223 and miR-146a in peripheral blood of acute ischemic stroke and their relationships with the occurrence of disease. Methods: 76 cases of acute ischemic stroke diagnosed and treated in the Fourth Affiliated Hospital of Harbin Medical University from January 2017 to April 2018 were selected as the subjects (case group), according to the TOAST etiological classification, there were 33 cases of main artery atherosis type, 19 cases of cardiogenic embolism type, 16 cases of small artery occlusion type, and 8 cases of unknown cause type. 60 healthy subjects were as the control group. The expression levels of miR-223 and miR-146a in peripheral blood were detected by RT-PCR, NIHSS score was used to assess the severity of acute ischemic stroke, the relationships between miR-223 and miR-146a levels with acute stroke were analyzed. Results: The expression level of miR-223 in peripheral blood of case group was significantly higher than that of control group (P<0.05), the expression level of miR-146a was significantly lower than that of the control group (P<0.05); there was no significant difference in the expression levels of miR-223 and miR-146a between the different etiological subtypes (P>0.05); there was a significant positive correlation between miR-233 level with NIHSS score in case group (r=0.774, P=0.000), there was a significant negative correlation between miR-146a level with NIHSS score (r=0.741, P=0.000); hypertension, hyperglycemia, platelet count, INR and miR-223 were risk factors for acute ischemic stroke, miR-146a is a protective factor for acute ischemic stroke. Conclusion: The miR-223 in peripheral blood of patients with acute ischemic stroke is high expressed, the miR-146a is low expressed, it is closely related to the severity of the patient's condition
杨媛媛, 孟南希, 张欣, 陈修芬. 外周血miR-223和miR-146a水平与急性缺血性脑卒中的相关性研究[J]. 河北医学, 2019, 25(3): 599-602.
YANG Yuanyuan, et al. Study on the Correlation between Peripheral Blood MiR-223 and MiR-146a Levels and Acute Ischemic Stroke. HeBei Med, 2019, 25(3): 599-602.
[1] 宇传华,罗丽莎,李梅,等.从全球视角看中国脑卒中疾病负担的严峻性[J].公共卫生与预防医学,2016,27(1):1~5. [2] Zeng X, Zhang X, Zou Q. Integrative approaches for predicting microRNA function and prioritizing disease-related microRNA using biological interaction, networks[J]. Briefings in Bioinformatics, 2016, 17(2):193~203. [3] 中华医学会神经病学分会.中国急性缺血性脑卒中诊治指南2014[J].中华神经科杂志,2015,48(4):246~257. [4] Dunning K. National Institutes of Health Stroke Scale[J]. Encyclopedia of Clinical Neuropsychology, 2011, 9(1):1714~1715. [5] 郝宁,吕广明.miRNA在中枢神经系统功能与疾病中的作用研究进展[J].神经解剖学杂志,2014,30(6):715~719. [6] Wang J, Bai X, Song Q, et al. miR-223 inhibits lipid deposition and inflammation by suppressing toll-like receptor 4 signaling in macrophages[J]. International Journal of Molecular Sciences, 2015, 16(10):24965~24982. [7] Chen Y, Song Y, Huang J, et al. Increased circulating exosomal miRNA-223 is associated with acute ischemic stroke:[J]. Frontiers in Neurology, 2017, 8(9913):57~61. [8] Wang Y, Zhang Y, Huang J, et al. Increase of circulating miR-223 and insulin-like growth factor-1 is associated with the pathogenesis of acute ischemic stroke in patients[J]. Bmc Neurology, 2014, 14(1):1~7. [9] 黄甫高,王春芳,韦颖,等.中国人群miR-146a基因rs2910164多态性与缺血性脑卒中易感性的meta分析[J].分子影像学杂志,2017,40(4):424~429. [10] Su Z F, Sun Z W, Zhang Y, et al. Regulatory effects of miR-146a/b on the function of endothelial progenitor cells in acute ischemic stroke in mice[J]. Kaohsiung Journal of Medical Sciences, 2017, 33(8):369~378.
2019, Vol. 25 
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