Abstract：Objective: To investigate the expressions of miR-144-3p and serum/glucocorticoid regulated protein kinase 3 (SGK3) in the oral squamous cell carcinoma (OSCC) tissues, and to analyze the relationships between the them with the clinicopathological feature and prognosis. Methods: 78 cases of OSCC tissue specimens were selected as case group, and normal oral mucosa epithelium specimens were taken as control group, real time quantitative PCR (RT-PCR) was used to detect the relative expressions of miR-144-3p and SGK3 in tissues, Western blot and immunohistochemistry were used to detect the SGK3 protein expression in tissues, the relationships between the them with clinicopathological features and prognosis were analyzed. The working characteristic curve (ROC) of the subjects was made, evaluated the diagnostic efficiencies of miR-144-3p and SGK3 for OSCC; all patients were followed up for 30 months, the survival rate of patients was statistically analyzed, survival was analyzed by Kaplan-Meier, survival differences between the two groups were detected by Log-Rank. Pearson correlation analysis was used to analyze the correlation between miR-144-3p and SGK3. Results: Compared with the control group, the expression of miR-144-3p mRNA in OSCC group was increased, SGK3 mRNA, protein and protein positive expression were all decreased (P<0.05), miR-144-3p expression and SGK3 positive expression were not related to sex, age, smoking status and depth of tumor invasion (P>0.05), was related to TNM stage, tumor differentiation and lymph node metastasis (P<0.05). The ROC curve showed that the AUC of miR-144-3p in the prediction of OSCC was 0.767, the sensitivity was 71.79%, and the specificity was 63.96%. The AUC of SGK3 in the prediction of OSCC was 0.683, the sensitivity was 66.67%, and the specificity was 75.64%. Followed up for 30 months, 27 patients died, the survival rate was 65.38%. The mortality rates of miR-144-3p high expression group (44.68%) and SGK3 negative group (48.78%) were significantly higher than those of miR-144-3p low expression group (19.35%) and SGK3 positive group (18.92%), Kaplan-Meier survival curve showed that the overall survival rate of the two groups was significantly different (P<0.05). There was a significant negative correlation between miR-144-3p and SGK3 expression (r=-0.417, P<0.05). Conclusion: The expression of miR-144-3p in OSCC tissues is up-regulated, and the expression of SGK3 is down-regulate, it is related to the stage of tumor, the degree of differentiation, and lymph node metastasis, it may be a biological indicator of the diagnosis and prognosis of OSCC.
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2019, Vol. 25 
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